https://www.journals.psmpublishers.org/index.php/ijnas/issue/feed International Journal of Nanotechnology and Allied Sciences 2019-03-25T21:13:50+00:00 International Journal of Nanotechnology and Allied Sciences ijnas@psmpublishers.org Open Journal Systems <p>International Journal of Nanotechnology and Allied Sciences (IJNAS; ISSN: 2523-9252) is a peer-reviewed, open access,&nbsp; international journal that considers articles on all aspects of nanotechnology and allied sciences.</p> https://www.journals.psmpublishers.org/index.php/ijnas/article/view/283 Validation of the Ethnopharmacological Uses of Withania somnifera 2019-03-25T21:13:50+00:00 Muhammad Shuaib zeyadz44@yahoo.com <p style="text-align: justify;">The antimicrobial potential of some solvent extracts of <em>Withania somnifera</em> leaves against different strains of bacteria and fungi was investigated. The leaves of <em>W. somnifera</em> species were collected from Bacha Khan University, Charsadda, Pakistan, rinsed with distilled water and extracted with chloroform and ethanol. Antibacterial activity of crude extracts of <em>W. somnifera</em> was determined by agar well diffusion method against gram positive, gram negative bacterial and antifungal activity was determined by agar diffusion method against fungal strains. All tested plant extracts, showed varying zones of inhibition against bacteria and fungi tested. The highest zone of inhibition of 27 mm diameter was noted in <em>S. aureus</em> with ethanol extract while the lowest 7 mm zone of inhibition was reported in <em>Klebsiella pneumonia</em>. Among the fungal species, promising level of antifungal activity was observed in all fractions against <em>A. flavus</em> and <em>A. niger</em>. The results obtained exhibit that this plant has good medicinal potential, and it needs further phytochemical exploitation to isolate phytochemical constituents having antibacterial and antifungal activities.</p> 2019-01-31T00:00:00+00:00 Copyright (c) 2019 PSM https://www.journals.psmpublishers.org/index.php/ijnas/article/view/285 Direct Regeneration from Leaf Disc Explants of Peanut: Grafting Improves Survival Rate 2019-03-25T21:03:44+00:00 Mahmood ul Hassan hmhassanq@hotmail.com <p style="text-align: justify;">Leaf discs of 0.5 cm diameter from 10 days old seedlings of four peanut cultivars were cultured on Murashige and Skoog (MS) medium supplemented with twelve different combinations of Thidiazuron (TDZ) and Naphthalene acetic acid (NAA). Highest number of responding explants (52.67%) with highest number of shoot buds (5.65) per responding explant was achieved at a combination of 0.5 mg/l NAA and 0.5 mg/l TDZ. The explants were then shifted to fresh MS medium containing 8 mg/l Benzylaminopurine (BAP) and 0.5 mg Indole Acetic Acid (IAA) for shoot elongation. Root induction was highest (56.07%) at half strength MS medium supplemented with 1.5 mg/l NAA. Grafting of in vitro regenerated shoots on 10 days old seedling root stock showed highest (61.30%) success rate. Survival rate of plants obtained from grafting was almost double (45.76%) than those achieved by rooting of shoots (22.41%). There was significant variation among four varieties regarding number of responding explant, number of shoots per explant, rooting or grafting percentage and survival rate. Golden proved to be the best variety in terms of number of plant reaching to maturity.</p> 2019-01-31T00:00:00+00:00 Copyright (c) 2019 PSM