The Antioxidant and antibacterial activities of the species Silene inflata Sm.

biological activities of S. inflata

  • soumia mouffouk, Dr University of batna-1 algeria https://orcid.org/0000-0001-7704-4093
  • chaima mouffouk, Dr University of batna-1 algeria
  • Leila Hambaba University of batna-2 algeria
  • sara mouffouk, Dr University of batna-2 algeria
  • Samia Dekkiche, Dr University of batna-2 algeria
Keywords: Silene inflata, phenolic content, phytochemical screening, antioxidant, antibacterial activity.

Abstract

This study is devoted to the estimation of total phenolic content and the evaluation of in vitro antioxidant and antibacterial activities of crude extracts (petroleum ether, ethyl acetate and methanolic) obtained from the species Silene inflata Sm. The total phenolic content of the obtained extracts was estimated spectrophotometrically by Folin-Ciocalteu method, the antioxidant activity was determinated by scavenging of the free radical DPPH. Furthermore, the antibacterial activity was assessed by agar disk diffusion assay against four bacterial strains. The results of the phytochemical screening of crude extracts revealed the presence of several types of secondary metabolites including: steroids, alkaloids, tannins, polyphenols and saponins. The ethyl acetate extract recorded the highest content of polyphenols (8.60 ± 0.01 µg GAE/mg of plant extract) followed by the methanolic and petroleum ether extracts respectively. The results of the antioxidant activity showed that all the tested extracts had the ability to scavenge the free radical DPPH and possess an antioxidant activity in a dose-dependent manner. The methanolic extract has a strong antioxidant activity with an IC50 value of 23.53 ± 0.15 μg/mL. The crude extracts (petroleum ether and ethyl acetate) had no antibacterial effect against all the tested strains compared to penicillin and gentamicin as positive controls. While the methanolic extract revealed an antibacterial effect only against the clinical strain Staphylococcus albus with a value of the minimum inhibitory concentration at 0.5 mg/ml. In conclusion, the species Silene inflata Sm. could be an important source of new therapeutic agents against pathological damage due to free radicals and microbial infections.

Author Biographies

soumia mouffouk, Dr, University of batna-1 algeria

Laboratoire de Chimie et Chimie de l’Environnement (L.C.C.E), Département de Chimie, Faculté des Sciences de la Matière, Université de Batna-1, Algérie

chaima mouffouk, Dr, University of batna-1 algeria

Laboratoire de chimie des matériaux et des vivants: activité et réactivité (LCMVAR) Université de Batna-1, Algérie

Leila Hambaba, University of batna-2 algeria

Laboratoire de chimie des matériaux et des vivants: activité et réactivité (LCMVAR) Université de Batna-1, Algérie

sara mouffouk, Dr, University of batna-2 algeria

Laboratoire de chimie des matériaux et des vivants: activité et réactivité (LCMVAR) Université de Batna-1, Algérie

Samia Dekkiche, Dr, University of batna-2 algeria

Laboratoire de chimie des matériaux et des vivants: activité et réactivité (LCMVAR) Université de Batna-1, Algérie

Published
2019-04-28
How to Cite
mouffouk, soumia, mouffouk, chaima, Hambaba, L., mouffouk, sara, & Dekkiche, S. (2019). The Antioxidant and antibacterial activities of the species Silene inflata Sm. PSM Biological Research, 4(2), 74-86. Retrieved from https://www.journals.psmpublishers.org/index.php/biolres/article/view/262
Section
Articles